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True/False MCQ · Genital Tract and Sexually Transmitted Infections

Concerning the laboratory diagnosis of sexually transmitted infections, the following statements are correct:

Decide whether each statement is true or false.

  1. ANAAT is used to diagnose Chlamydia trachomatis and Neisseria gonorrhoeae
  2. BAn endocervical swab is inappropriate for diagnosing gonococcal cervicitis
  3. CA self-collected vaginal swab is an acceptable specimen for NAAT
  4. DFirst-void (voided) urine in men can be used for chlamydia/gonorrhoea NAAT
  5. EDark-ground microscopy is the test of choice for chlamydia
Show answers and explanation
  • A. True
  • B. False: An endocervical swab is appropriate for gonococcal cervicitis (gonorrhoea causes cervicitis).
  • C. True
  • D. True
  • E. False: Dark-ground microscopy is for syphilis, not chlamydia (which is diagnosed by NAAT).

Laboratory diagnosis of STIs

NAAT and specimens
  • NAAT is the test of choice for Chlamydia trachomatis and Neisseria gonorrhoeae.
  • Acceptable specimens: cervical/urethral swabs, first-void (voided) urine in men, and self-collected vaginal swabs.
  • Gonorrhoea causes cervicitis, so an endocervical swab IS appropriate (a posterior-fornix high vaginal swab is for vaginitis).
Other tests

Culture and serology are also used; dark-ground microscopy is for syphilis, not chlamydia.

Why A is true

NAAT diagnoses chlamydia and gonorrhoea.

Why B is false

An endocervical swab is appropriate for gonococcal cervicitis.

Why C is true

A self-collected vaginal swab is acceptable for NAAT.

Why D is true

First-void urine in men is acceptable for NAAT.

Why E is false

Dark-ground microscopy is for syphilis, not chlamydia.

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